Journal: Drug Design, Development and Therapy

Article Title: Network Pharmacology and Molecular Docking Study of the Chinese Miao Medicine Sidaxue in the Treatment of Rheumatoid Arthritis

doi: 10.2147/dddt.s330947

Figure Lengend Snippet: Figure 11 Docking results of the main active compounds of SX and the key RA-associated targets. (A1) TNF-α and β-sitosterol; (A2) TNF-α and 7-oxo-β-sitosterol; (A3) TNF-α and (−)-catechin gallate; (A4) TNF-α and periplogenin. (B1) VEGF and cajanin; (B2) VEGF and (−)-catechin gallate. (C1) STAT1 and eleutheroside A; (C2) STAT1 and β-sitosterol; (C3) STAT1 and (−)-catechin gallate. (D1) IL-6 and cajanin; (D2) IL-6 and licochalcone A. (E1) PTGS2 and cajanin; (E2) PTGS2 and medicarpin. (F1) IL-2 and eleutheroside A; (F2) IL-2 and calycosin. (G1) NF-κB and (−)-catechin gallate; (G2) NF-κB and medicarpin. (H1) AKT and β-sitosterol-3-O-β-D-glucopyranoside. (H2) AKT and β-sitosterol; (H3) AKT and (-)-catechin gallate. (I1) PI3K and eleutheroside A;(I2) PI3K and β-sitosterol; (I3) PI3K and (−)-catechin gallate.

Article Snippet: Rat vascular endothelial growth factor (VEGF-A), TNF-α, IL-2, IL-6 enzyme-linked immunoassay (ELISA) kit (MultiScience), PI3 Kinase p110α (C73F8) Rabbit mAb(#4249), Akt Antibody (#9272), Phospho-Akt (Ser473) (193H12) Rabbit mAb (#4058) and NF-κB p65 (D14E12) XP Rabbit mAb (#8242), Phospho-NF -κB p65 (Ser536) (93H1) Rabbit mAb(#3033) were purchased from Cell Signaling (Danvers, MA, USA).

Techniques:

Journal: Drug Design, Development and Therapy

Article Title: Network Pharmacology and Molecular Docking Study of the Chinese Miao Medicine Sidaxue in the Treatment of Rheumatoid Arthritis

doi: 10.2147/dddt.s330947

Figure Lengend Snippet: Figure 14 The effect of SX on the rat’s serum levels of IL-2, TNF-α, VEGF-A and IL-6 (Mean ± SEM, n = 10). (A) IL-2: *Compared to the Nor group, *P<0.05, (Mod group vs Nor group, *P=0.0215); #Compared to the Mod group, #P<0.05, ##P<0.01 (GTW group vs Mod group, #P=0.029; SX 40g/kg group vs Mod group, #P=0.021); ▲Compared to the GTW group, ▲P<0.05 (Mod group vs GTW group, ▲P=0.0298;SX 10g/kg group vs GTW group, ▲P= 0.0381). (B) TNF-α: *Compared to the Nor group, **P<0.01,(Mod group vs Nor group,**P=0.0064); #Compared to the Mod group, #P<0.05, ##P<0.01 (GTW group vs Mod group, #P=0.0346;SX 40g/kg group vs Mod group, #P= 0.0275, SX 20g/kg group vs Mod group, #P=0.0167); ▲Compared to the GTW group, ▲P<0.05 (Mod group vs GTW group, ▲P=0.0273; SX 10g/kg group vs GTW group, ▲P=0.0219). (C) VEGF-A: *Compared to the Nor group, **P<0.01, (Mod group vs Nor group,**P=0.0024); #Compared to the Mod group, #P<0.05, ##P<0.01 (GTW group vs Mod group, ##P=0.0062;SX 40g/kg group vs Mod group, ##P=0.0039, SX 20g/kg group vs Mod group, #P=0.0273); ▲Compared to the GTW group, ▲P<0.05 (Mod group vs GTW group, ▲P=0.0124;SX 10g/kg group vs GTW group, ▲P=0.0318). (D) IL-6: *Compared to the Nor group, **P<0.01,(Mod group vs Nor group, **P=0.0078); #Compared to the Mod group, #P<0.05, ##P<0.01 (GTW group vs Mod group, ##P=0.0043;SX 40g/kg group vs Mod group, ##P=0.0059, SX 20g/kg group vs Mod group, #P=0.0357); ▲Compared to the GTW group, ▲P<0.05 (Mod group vs GTW group, ▲P=0.0415; SX 10g/kg group vs GTW group, ▲P=0.0371). Abbreviations: CIA, collagen-induced arthritis; GTW, Tripterygium wilfordii polyglycoside tablet; SX, Sidaxue.

Article Snippet: Rat vascular endothelial growth factor (VEGF-A), TNF-α, IL-2, IL-6 enzyme-linked immunoassay (ELISA) kit (MultiScience), PI3 Kinase p110α (C73F8) Rabbit mAb(#4249), Akt Antibody (#9272), Phospho-Akt (Ser473) (193H12) Rabbit mAb (#4058) and NF-κB p65 (D14E12) XP Rabbit mAb (#8242), Phospho-NF -κB p65 (Ser536) (93H1) Rabbit mAb(#3033) were purchased from Cell Signaling (Danvers, MA, USA).

Techniques:

Journal: Drug Design, Development and Therapy

Article Title: Network Pharmacology and Molecular Docking Study of the Chinese Miao Medicine Sidaxue in the Treatment of Rheumatoid Arthritis

doi: 10.2147/dddt.s330947

Figure Lengend Snippet: Figure 15 The effect of SX on the mRNA levels of NF-κBp65, STAT1, PTGS2, PI3K, AKT and VEGF-A in the rat’s joint synovial tissue (Mean ± SEM, n = 10). (A) NF-κBp65: *Compared to the Nor group, **P<0.01 (Mod group vs Nor group, **P=0.0064); #Compared to the Mod group, #P<0.05, ##P<0.01 (SX 40g/kg group vs Mod group, #P=0.026; SX 20g/kg group vs Mod group, #P=0.023; GTW group vs Mod group, ##P=0.0076); ▲Compared to the GTW group, ▲▲P<0.01 (Mod group vs GTW group, ▲▲P=0.0058; SX 10g/kg group vs GTW group, ▲▲P=0.0072). (B) STAT1: *Compared to the Nor group, **P<0.01 (Mod group vs Nor group, **P=0.0018); #Compared to the Mod group, #P<0.05, ##P<0.01 (SX 20g/kg group vs Mod group, #P=0.031; GTW group vs Mod group, ##P=0.0085; SX 40g/kg group vs Mod group, ##P=0.0038); ▲Compared to the GTW group,▲P<0.05, ▲▲P<0.01 (SX 20g/kg group vs GTW group, ▲P=0.041; Mod group vs GTW group, ▲▲P=0.0089;SX 10g/kg group vs GTW group, ▲▲P= 0.0068). (C) PTGS2: *Compared to the Nor group, **P<0.01 (Mod group vs Nor group, **P=0.0081); #Compared to the Mod group, #P<0.05, ##P<0.01 (SX 40g/kg group vs Mod group, #P=0.036; GTW group vs Mod group, ##P=0.0067); ▲Compared to the GTW group, ▲P<0.05, ▲▲P<0.01 (SX 20g/kg group vs GTW group, ▲P=0.023; Mod group vs GTW group, ▲▲P=0.0049; SX 10g/kg group vs GTW group, ▲▲P= 0.0037). (D) PI3K: *Compared to the Nor group, **P<0.01 (Mod group vs Nor group, **P=0.0074); #Compared to the Mod group, #P<0.05, ##P<0.01 (SX 20g/kg group vs Mod group, #P=0.024; GTW group vs Mod group, ##P=0.0088; SX40g/kg vs Mod, ##P=0.0049); ▲Compared to the GTW group, ▲P<0.05, ▲▲P<0.01 (SX 10g/kg group vs GTW group, ▲P=0.031; Mod vs GTW, ▲▲P=0.0073). (E) AKT: *Compared to the Nor group, **P<0.01 (Mod group vs Nor group, **P=0.0091); #Compared to the Mod group, #P<0.05, ##P<0.01 (SX 20g/kg group vs Mod group, #P=0.0042; GTW group vs Mod group, ##P=0.0095; SX 40g/kg group vs Mod group, ##P=0.0056); ▲Compared to the GTW group, ▲P<0.05, ▲▲P<0.01 (SX 20g/kg group vs GTW group, ▲P=0.0431; Mod group vs GTW group, ▲▲P=0.0072; SX 10g/kg group vs GTW group, ▲▲P=0.0075). (F) VEGF-A: *Compared to the Nor group, **P<0.01 (Mod group vs Nor group, **P=0.0093); #Compared to the Mod group, #P<0.05, ##P<0.01 (SX 40g/kg group vs Mod group, #P=0.039; SX 20g/kg group vs Mod group, #P= 0.028; GTW group vs Mod group, ##P= 0.0075; SX 10g/kg group vs Mod group, #P=0.039; SX 10g/kg group vs Mod group, #P=0.028); ▲Compared to the GTW group, ▲P<0.05, ▲▲P<0.01 (SX 10g/kg group vs GTW group, ▲P=0.019; Mod group vs GTW group, ▲▲P=0.0035). Abbreviations: CIA, collagen-induced arthritis; GTW, Tripterygium wilfordii polyglycoside tablet; SX, Sidaxue.

Article Snippet: Rat vascular endothelial growth factor (VEGF-A), TNF-α, IL-2, IL-6 enzyme-linked immunoassay (ELISA) kit (MultiScience), PI3 Kinase p110α (C73F8) Rabbit mAb(#4249), Akt Antibody (#9272), Phospho-Akt (Ser473) (193H12) Rabbit mAb (#4058) and NF-κB p65 (D14E12) XP Rabbit mAb (#8242), Phospho-NF -κB p65 (Ser536) (93H1) Rabbit mAb(#3033) were purchased from Cell Signaling (Danvers, MA, USA).

Techniques:

Journal: Cell & Bioscience

Article Title: Maternal hyperhomocysteinemia induces fetal growth restriction by suppressing angiogenesis at the maternal-fetal interface

doi: 10.1186/s13578-025-01529-0

Figure Lengend Snippet: HHcy impairs vascular density at the maternal-fetal interface and affects angiogenic factor expression in decidua. A Representative images and quantification of CD31 immunofluorescence staining in decidua of ND and HMD pregnant rats at E10 ( n = 4 per group). Scale bar = 200 μm. B Representative images and expression of CD31 immunofluorescence staining in placentas at E14 ( n = 4 per group). Scale bar = 100 μm. C Representative images and expression of CD31 immunofluorescence staining in placentas at E20 ( n = 4 per group). Scale bar = 100 μm. D Protein expression levels of VEGFA, PLGF, HIF1α, and CA9 in decidua at E10 ( n = 6 per group). The data were expressed as mean ± SEM and statistical comparisons were assessed by unpaired two-tailed Student’s t-test. ns P > 0.05, * P < 0.05, ** P < 0.01

Article Snippet: VEGFA concentration in the CM of DSCs was measured using a rat VEGFA ELISA kit (Cusabio, CSB-E04757r, Wuhan, China) following the manufacturer’s instructions.

Techniques: Expressing, Immunofluorescence, Staining, Two Tailed Test

Journal: Cell & Bioscience

Article Title: Maternal hyperhomocysteinemia induces fetal growth restriction by suppressing angiogenesis at the maternal-fetal interface

doi: 10.1186/s13578-025-01529-0

Figure Lengend Snippet: HHcy inhibits angiogenesis by reducing VEGFA secretion from DSCs. A Cell viability of DSCs assessed by CCK8 assay after treatment with different concentrations of Hcy for 24 h ( n = 6 per group). B VEGFA mRNA expression in DSCs following treatment with varying concentrations of Hcy for 24 h ( n = 4 per group). C and D VEGFA protein levels in DSCs treated with normal culture medium (NC) or medium containing 4000 µM Hcy (HHcy) for 24 h ( n = 4 per group). E VEGFA mRNA levels in NC and HHcy groups ( n = 4 per group). F VEGFA concentration in DSCs culture supernatants, analyzed by ELISA ( n = 6 per group). G – I CM from DSCs was used for HUVECs tube formation assays. Images were taken 6 h post-seeding. Scale bar = 100 μm. Quantitative analysis of tube branch number and total tube length ( n = 3 per group). J and K CM from DSCs was used for HUVECs wound healing assays. Scratch images were taken at 0 and 12 h. Scale bar = 50 μm. Wound healing rates were quantified ( n = 3 per group). The data were expressed as mean ± SEM and statistical comparisons were assessed by unpaired two-tailed Student’s t-test. ns P > 0.05, * P < 0.05, *** P < 0.001, **** P < 0.0001

Article Snippet: VEGFA concentration in the CM of DSCs was measured using a rat VEGFA ELISA kit (Cusabio, CSB-E04757r, Wuhan, China) following the manufacturer’s instructions.

Techniques: CCK-8 Assay, Expressing, Concentration Assay, Enzyme-linked Immunosorbent Assay, Two Tailed Test

Journal: Cell & Bioscience

Article Title: Maternal hyperhomocysteinemia induces fetal growth restriction by suppressing angiogenesis at the maternal-fetal interface

doi: 10.1186/s13578-025-01529-0

Figure Lengend Snippet: HHcy inhibits VEGFA expression and secretion by upregulating CD36 expression in DSCs, thereby impairing angiogenesis. A The VEGFA mRNA expression of DSCs after treatment with Hcy and/or 100µM SSO ( n = 3 per group). B and C The VEGFA protein expression of DSCs after treatment with Hcy and/or 100µM SSO ( n = 3 per group). D VEGFA concentration in the culture supernatant of DSCs after treatment with Hcy and/or 100µM SSO, measured by ELISA ( n = 6 per group). E– G Conditioned medium (CM) collected from treated DSCs was used for HUVECs tube formation assays. Images were taken 6 h after incubation. Scale bar = 100 μm. Quantitative analysis of branch number and total tube length ( n = 3 per group). H CM from treated DSCs was used for HUVECs wound healing assays. Images were taken at 0 h and 12 h. Scale bar = 50 μm. The wound healing rate was quantified ( n = 3 per group). The data were expressed as mean ± SEM and statistical comparisons were assessed by unpaired two-tailed Student’s t-test. ns P > 0.05, * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001

Article Snippet: VEGFA concentration in the CM of DSCs was measured using a rat VEGFA ELISA kit (Cusabio, CSB-E04757r, Wuhan, China) following the manufacturer’s instructions.

Techniques: Expressing, Concentration Assay, Enzyme-linked Immunosorbent Assay, Incubation, Two Tailed Test

Journal: Cell & Bioscience

Article Title: Maternal hyperhomocysteinemia induces fetal growth restriction by suppressing angiogenesis at the maternal-fetal interface

doi: 10.1186/s13578-025-01529-0

Figure Lengend Snippet: Schematic diagram of this study. HHcy leads to lipid deposition in decidua by upregulating CD36 expression in DSCs, which suppresses VEGFA expression and secretion possibly by activating the PPAR signaling pathway, thereby impairing angiogenesis at the maternal-fetal interface and causing FGR. The diagram was created at https://BioRender.com

Article Snippet: VEGFA concentration in the CM of DSCs was measured using a rat VEGFA ELISA kit (Cusabio, CSB-E04757r, Wuhan, China) following the manufacturer’s instructions.

Techniques: Expressing

Journal: Archives of Medical Science : AMS

Article Title: MALAT1/miR-320a in bone marrow mesenchymal stem cells function may shed light on mechanisms underlying osteoporosis

doi: 10.5114/aoms/105838

Figure Lengend Snippet: A – HE staining results of the tibial metaphyseal bone obtained from ovariectomy and sham surgery rats ( n = 24). B – Images from micro-CT scanning of the proximal tibia in osteoporotic and intact SD rats; a significant reduction in the number of trabeculae and a decrease in the continuity of bone trabeculae can be seen in the osteoporosis group. C , D – Expression levels of miR-320a and MALAT1 were determined by qRT-PCR, and significantly reduced expression of MALAT1 and overexpression can be seen in Figure 1 C and 1 D. E – Protein expression of NRP-1 and β-catenin was determined by immunohistochemistry. Scale bar = 20 µm (* p < 0.05, ** p < 0.01 compared with control)

Article Snippet: Then, sections were washed 3 times with PBS (5 min each time), dried and blocked in 5% BSA for 20 min. After the removal of BSA liquid, 50 μl of primary antibody diluted against NRP-1 and β-catenin (rat polyclonal anti-NRP-1 antibody 1 : 250, rat polyclonal anti-β-catenin antibody 1 : 500, Boster, Wuhan, China) was added to each section, and the tissues was covered at 4°C overnight.

Techniques: Staining, Micro-CT, Expressing, Quantitative RT-PCR, Over Expression, Immunohistochemistry, Control

Journal: Archives of Medical Science : AMS

Article Title: MALAT1/miR-320a in bone marrow mesenchymal stem cells function may shed light on mechanisms underlying osteoporosis

doi: 10.5114/aoms/105838

Figure Lengend Snippet: Number of NRP1-positive and β-catenin-positive cells after immunohistochemistry in the tibia (1000×)

Article Snippet: Then, sections were washed 3 times with PBS (5 min each time), dried and blocked in 5% BSA for 20 min. After the removal of BSA liquid, 50 μl of primary antibody diluted against NRP-1 and β-catenin (rat polyclonal anti-NRP-1 antibody 1 : 250, rat polyclonal anti-β-catenin antibody 1 : 500, Boster, Wuhan, China) was added to each section, and the tissues was covered at 4°C overnight.

Techniques: Immunohistochemistry, Control

Journal: Archives of Medical Science : AMS

Article Title: MALAT1/miR-320a in bone marrow mesenchymal stem cells function may shed light on mechanisms underlying osteoporosis

doi: 10.5114/aoms/105838

Figure Lengend Snippet: A – Protein level of β-catenin in the cytoplasm and nucleus was determined by Western blot; β-catenin expression was observed to be significantly decreased in MALAT1 siRNA and miR-320a mimics groups in the nucleus. B – The ratio of β-catenin in the nucleus to that in the cytoplasm was calculated and compared between groups (** p < 0.01 compared with control). C – F – Western blot analysis was used to identify the osteogenic differentiation of BMSCs in different groups, by evaluation of the protein levels of NRP-1, OCN, and OPN (NRP-1 – neuropilin-1; OCN – osteocalcin; OPN – osteopontin) (* p < 0.05, ** p < 0.01 compared with control)

Article Snippet: Then, sections were washed 3 times with PBS (5 min each time), dried and blocked in 5% BSA for 20 min. After the removal of BSA liquid, 50 μl of primary antibody diluted against NRP-1 and β-catenin (rat polyclonal anti-NRP-1 antibody 1 : 250, rat polyclonal anti-β-catenin antibody 1 : 500, Boster, Wuhan, China) was added to each section, and the tissues was covered at 4°C overnight.

Techniques: Western Blot, Expressing, Control